EN 14104 Fat and oil derivatives - Fatty Acid Methyl Esters (FAME) - Determination of acid value
1 Scope
This European Standard specifies one titrimetric method for the determination of acid value in light coloured Fatty Acid Methyl Esters, hereinafter referred as FAME.
It allows the determination of acid value within a range of 0.10 mg KOH/g to 1.00 mg KOH/g.
2 Normative references
This European Standard incorporates by dated or undated reference, provisions from other publications. These normative references are cited at the appropriate places in the text, and the publications are listed hereafter. For dated references, subsequent amendments to or revisions of any of these publications apply to this European Standard only when incorporated in it by amendment or revision. For undated references the latest edition of the publication referred to applies (including amendments).
EN ISO 661, Animal and vegetable fats and oils - Preparation of test sample (ISO 661:1989).
EN ISO 3696, Water for analytical laboratory use - Specification and test methods (ISO 3696:1987).
3 Terms and definitions
For the purposes of this European Standard, the following term and definition apply.
3.1 acid value
number of milligrams of potassium hydroxide required to neutralise the free fatty acids present in 1 g of FAME, when determined in accordance with the procedure specified in this European Standard.
Acid value is expressed in milligrams of potassium hydroxide per gram of sample.
NOTE If the sample contains mineral acids these are, by convention determined as a part of total acid value. This method does not allow to distinguish between weak (from free fatty acids) and strong (from mineral acids, if present) acidity.
4 Principle
A test portion is dissolved in a mixed solvent and titrated with a diluted solution of potassium hydroxide, using phenolphthalein as an indicator in order to detect the titration end point.
