3 Sampling

Oil samples are collected in accordance with IEC 475 and IEC 567. Samples should be protected from direct light.

4 Labelling of samples

Samples shall be labelled in accordance with IEC 567.

5 Apparatus

5.1 Genearl equipment:

- laboratory glassware;

- beakers or Erlenmeyer flasks;

- volumetric flasks;

- pipettes;

- analytical balance (0.1mg)

5.2 Equipment for extraction by method A

- 25mL glass stoppered graduated cylinder;

- 5mL pipette;

- mechanical shaker.

5.3 Equipment for extraction by method B

- silica cartirdges, containing about 1g of silica with a particle size ranging from 15um to 100um. Commercially available cartridges are suitable;

- a vacuum device suitable to produce a slight vacuum (i.e. filter pump or vacuum manifold);

- a 10mL luer lock syringe;

- volumetric flasks, 2mL.

5.4 High-performance liquid chromatograph(HPLC)

A high-performance liquid chromatograph equipped with:

- pumping system suitable for at least two solvents;

- injection device capable of injecting 10uL to 20uL and suitable precision syringe(50uL to 250uL);

- reversed phase column packed with octyl or octadecyl bonded silica;

- pre-column packed with teh same material as the analytical column (optional);

- UV detector capable of recording UV spectra; a diode array UV detector is recommended;

- data acquisition system (recorder or integrator).