ASTM D6731 Standard Test Method for Determining the Aerobic, Aquatic Biodegradability of Lubricants or Lubricant Components in a Closed Respirometer
14. Report
14.1 Report the following data and information:
14.1.1 Information on the inoculum, including source, date of collection, storage, handling, and if used, the method of pre-adaptation to the test material.
14.1.2 Method and results of determination of microorganisms.
14.1.3 Identification of the reference material.
14.1.4 Elemental analysis (giving percentage composition of all elements present), or empirical formula used to derive ThO2 of the test material and reference material.
14.1.5 Information on preparation of the test material and reference material, including any procedures for enhancing their dispersion into the test medium.
14.1.6 Main characteristics of the respirometer (manufacturer's name and address where appropriate).
14.1.7 Percentage of ThO2 achieved at the plateau and the number of days to reach the plateau.
14.1.8 Percentage of ThO2 achieved for each test material and reference material by the end of the test and the length of the test.
14.1.9 The cumulative average of percent ThO2 over time should be displayed graphically for each test and reference material because the lag-phase, that is, the delay in the onset of biodegradation as well as the rate of biodegradation are important. It is optional to graphically plot the percentage of ThO2 achieved over time for the individual replicates.
14.1.10 The replicate standard deviation (if applicable) for each test and reference material. The average of all replicates unless one or more replicates may be excluded based on statistical grounds as given in ISO 4259:1992(E). In that case, report the excluded data and the reason for exclusion.
14.1.11 The temperature range of the test.
14.1.12 Initial and final pH.
15. Precision and Bias
15.1 The precision and bias of the procedure in this test method for measuring the aerobic aquatic biodegradability of lubricants or their components is not determined as of yet.
16. Keywords
16.1 aerobic; biodegradation; BOD; degree (of biodegradation); municipal; respirometer; sewage sludge; ThO2; water insoluble
