ASTM D6731 Standard Test Method for Determining the Aerobic, Aquatic Biodegradability of Lubricants or Lubricant Components in a Closed Respirometer
10. Hazards
10.1 This test method includes the use of hazardous chemicals. Avoid contact with chemicals and follow the manufacturer's instructions and Material Safety Data Sheets.
10.2 This test method includes the use of potentially harmful microorganisms. As such, execution of this test method must be carried out under the guidance of qualified personnel who understand the safety and health aspects of working with microorganisms. Minimally, review this test method with an industrial hygienist before initiating any activity. Avoid contact with the microorganisms by using gloves and other appropriate protective equipment and sterile procedures. Use good personal hygiene to minimize exposure to harmful microbial agents.
10.3 Whenever appropriate, materials and supplies contaminated with biologically active cultures should be sterilized or autoclaved before discarding or reusing them.
10.4 Chemicals should be disposed ofas described in Guide D4447 or as prescribed by current regulations.
11. Preparation of Apparatus
11.1 Cleaning - The following is a suggested method for cleaning glassware and equipment to avoid organic contamination which may affect test results. The flasks and equipment used to prepare and store stock solutions and test solutions should be cleaned before use. Items should be washed with detergent and rinsed with water, a water-miscible organic solvent, water, acid (such as 10 % concentrated hydrochloric acid), and at least twice with distilled deionized water. Some organic solvents might leave a film that is insoluble in water. The presence of this film is not acceptable and may lead to false positive results. At the end of every test, all items that are to be used again should be immediately (a) emptied, (b) rinsed with water, and (c) cleaned as previously stated.
11.2 Test Environment - Incubation shall take place in the dark or in diffused light, in an enclosure that is maintained at a constant temperature (within +/- 1°C) between 20 and 24°C and which is free from toxic vapors.
