ASTM D6139 Aerobic Aquatic Biodegradation of Lubricants or Their Components
ASTM D6139 Standard Test Method for Determining the Aerobic Aquatic Biodegradation of Lubricants or Their Components Using the Gledhill Shake Flask
14. Report
14.1 Report the following data and information:
14.1.1 Information on the inoculum, including source, date of collection, storage, handling, and if used, the method for pre-adaptation to the test material.
14.1.2 Method and Results of Biomass Determination:
14.1.2.1 Viable microorganisms per millilitre in the inoculum at the beginning of the test, or
14.1.2.2 Sludge dry-weight expressed as milligrams solids per mL mixed liquor.
14.1.3 Identification of the reference material.
14.1.4 Carbon content of the test and reference materials.
14.1.5 Method for determining carbon content if Test Methods D5291 was not used.
14.1.6 Information on preparation of the test material and reference material, including any procedures for enhancing their dispersion into the test medium.
14.1.7 Percent of theoretical CO2 accumulated at the plateau and the number of days to reach the plateau for each test and reference material. If the plateau is reached prior to 28 days, the percent of theoretical CO2 accumulated at the end of 28 days should be reported as well.
14.1.8 Cumulative average percent of theoretical CO2 over time until the end of the study should be reported in tabular form for each test and reference material.
14.1.9 The cumulative average percent of theoretical CO2 over time should be displayed graphically for each test and reference material because the lag-phase, that is, delay in the onset of biodegradation, as well as the rate of biodegradation are important. It is optional to graphically plot the percentage of theoretical CO2 over time for the individual replicates.
14.1.10 The replicate standard deviation (if applicable) for each test material and reference material evaluated.
14.1.11 The average of all replicates unless one or more replicates may be excluded based on statistical grounds as given in ISO 4259. In that case, report the excluded data and the reason for exclusion.
14.1.12 The temperature range of the test.
14.1.13 Initial and final pH.
15. Precision and Bias
15.1 The precision and bias of the procedure in this test method for measuring the aerobic aquatic biodegradability of lubricants or their components is being determined.
16. Keywords
16.1 aerobic biodegradation; aquatic biodegradation; degree of biodegradation; lubricant biodegradability; municipal sewage; sewage; sludge; theoretical CO2 evolution
