ASTM D3606 Standard Test Method for Determination of Benzene and Toluene
ASTM D3606 Standard Test Method for Determination of Benzene and Toluene in Finished Motor and Aviation Gasoline by Gas Chromatography
5. Apparatus
5.1 Chromatograph - Any chromatographic instrument that has a backflush system and thermal conductivity detector, and that can be operated at the conditions given in Table 1, can be employed. Two backflush systems are shown. Fig. 1 is a pressure system and Fig. 2 is a switching valve system. Either one can be used.

5.2 Columns:
5.2.1 Column A - One 0.8-m (2.5-ft) by 3.2-mm (1/8-in.) outside diameter stainless steel column packed with 10 mass % dimethylpolysiloxane (for example, OV-101) on Chromosorb W, 60 to 80 mesh.

5.2.2 Column B - One 4.6-m (15-ft) by 3.2-mm outside diameter stainless steel column packed with 20 mass % TCEP on Chromosorb P, 80 to 100 mesh.

5.3 Recorder, a strip chart recorder. An electronic integrating device or a computer capable of graphical presentation of the chromatogram. The electronic integrating device or computer must be capable of measuring 0.1 volume % MEK with satisfactory signal-to-noise. If a strip chart recorder is to be used, a 0 to 1–mV range recording pontentiometer with a response time of 2 s or less and a maximum noise level of more or less 0.3 % of full scale is recommended. The detector strip chart recorder combination must produce a 4–mm deflection for a 2–uL sample containing 0.1 volume % MEK when operated at maximum sensitivity.

5.4 Microsyringe, 5-µL capacity.

5.5 Pipets, volumetric, 1 and 2-mL, calibrated in 0.01 mL; 5, 10, and 20-mL capacity.

5.6 Flasks, volumetric, 25 and 100-mL capacity.

5.7 Vibrator, electric.

5.8 Vacuum Source.

5.9 Evaporator, vacuum, rotary.

5.10 Flask, boiling, round-bottom, short-neck, with 24/40 standard taper joint, 500-mL capacity. Suitable for use with evaporator (5.9).

5.11 Lamp, infrared.

5.12 Burets, automatic, with integral reservoir, 25-mL capacity.

6. Materials
6.1 Carrier Gas - Helium, 99.99 % pure. (Warning - See Note 1.)

NOTE 1 - Warning: Compressed gas under high pressure.

6.2 Support - Crushed firebrick, acid-washed, 60 to 80-mesh and 80 to 100-mesh.

6.3 Liquid Phases - 1,2,3-Tris(2-cyanoethoxy) propane (TCEP) and methyl silicone.

6.4 Solvents:
6.4.1 Methanol, reagent grade. (Warning - See Note 2.)

NOTE 2 - Warning: Flammable. Vapor harmful. Can be fatal or cause blindness if swallowed or inhaled.

6.4.2 Chloroform, reagent grade. (Warning - See Note 3.)

NOTE 3 - Warning: Can be fatal if swallowed. Harmful if inhaled.

6.4.3 Methylene Chloride, for cleaning columns. (Warning - See Note 4.)

NOTE 4 - Warning: Harmful if inhaled. High concentrations can cause unconsciousness or death.

6.4.4 Acetone, for cleaning columns. (Warning - See Note 5.)

NOTE 5 - Warning: Extremely flammable. Vapors can cause flash fires.

6.5 Internal Standard:
6.5.1 Methyl Ethyl Ketone (MEK), 99.9% pure. (Warning - See Note 6.)

NOTE 6 - Warning: Flammable. Vapor can be harmful.

6.6 Calibration Standards:
6.6.1 Benzene, 99 + mol %. (Warning - See Note 7.)

NOTE 7 - Warning: Poison. Carcinogen. Harmful or fatal if swallowed. Extremely flammable. Vapors can cause flash fires.

6.6.2 Isooctane, 99 + mol %. (Warning - See Note 8.)

NOTE 8 - Warning: Extremely flammable. Harmful if inhaled.

6.6.3 Toluene. (Warning - See Note 9.)

NOTE 9 - Warning: Flammable. Vapor harmful.

6.6.4 n-Nonane, 99 + mol %. (Warning - See Note 10.)

NOTE 10 - Warning: Flammable. Vapor harmful.

7. Sampling
7.1 Gasoline. (Warning - See Note 11.) Samples to be analyzed by this test method shall be obtained using the procedures outlined in Practice D4057.

NOTE 11 - Warning: Extremely flammable. Vapors harmful if inhaled.